HatchTech B.V. is a Dutch technology company serving global food producers. Its Research Manager, Dr. Carla van der Pol, is using hyperspectral imaging and Raman spectroscopy to develop a commercially applicable optical technique for in-ovo sexing. Van der Pol’s team (including experts from Wageningen University and Research, Adaptation Physiology Group) is extracting small droplets of the embryos’ allantoic fluid from the egg at the eighth day of incubation using existing fluid uptake machinery and analyzing it through spectral technologies. The light’s wavelength patterns reveal whether the developing chick is male or female. Eggs containing a male embryo are then removed from the incubation process and can be used in other ways, while female embryos are returned to the incubator until hatch. This process is harmless, safe, pain free for the embryos, and it does not require consumables or long waiting times for the eggs between measurements and egg sorting.